"Visualizing proteins is crucial for understanding normal cell function, yet current labeling methods are limited. Despite their remarkable specificity, GFP-type proteins are too bulky, often exceeding the size of their protein targets—thus interfering with their function and trafficking—and the only small peptide label in use is toxic to cells. To address this, Alice Ting from the Massachusetts Institute of Technology and colleagues developed an enzyme-mediated intracellular protein label that’s small, highly specific, and not toxic.
Read more:Light traffic - The Scientist - Magazine of the Life Scienceshttp://www.the-scientist.com/2010/10/1/67/2/#ixzz11VkvWeqX"
C. Uttamapinant et al., “A fluorophore ligase for site-specific protein labeling inside living cells,”Proc Natl Acad Sci USA, 107:10914-19, 2010.Free F1000 Evaluation